Posted in 2 SBI4U Biology

SBI4U Gel Electrophoresis

Gel Electrophoresis Handouts

Go through this virtual gel electrophoresis lab to see how a gel is set up and run.

Go to the DNA Interactive site to complete the gel electrophoresis worksheets.

The final activity of the worksheet is to generate results of a gel from restriction enzyme digestions of lambda phage DNA (it’s a single chromosome that’s about 48 kb in length).  Lambda phage are viruses that infect and kill bacteria.  These have been used in molecular research.  You can learn about the virus cycles here.

Research and record the DNA palindromic recognition sites for each of the enzymes used in digesting the lambda DNA.  Record these beside the enzyme names on digestion map on your assignment. You can find them by searching the New England Biolabs website.

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